Special Topics in Molecular Biology and Genοmics – Τμήμα Πληροφορικής με εφαρμογές στην Βιοιατρική

Course ID

8ΕΒ09

Επίπεδο

Undergraduate

Είδος

Optional (compulsory)

Εξάμηνο

Περίοδος

Spring Semeter

ECTS

Ώρες Θεωρίας

Ώρες Εργαστηρίου

Instructor

Braliou Georgia

Description

Transcription in prokaryotic cells. Transcription in eukaryotes. Regulation of transcription and protein expression (promoters, enhancers, general and specific transcription factors, epigenetic modifications). Molecular Biology Techniques (cloning, types of PCR, DNA sequencing, genetic fingerprint). RNA interference regulates the function of genes, microRNAs. Genomics: fundamental principles of sequencing at the genome level (strategies, multiple coverage, contigs assembly, libraries, sequencing technologies, algorithms). Comparison and analysis of genomes (public databases, phylogenetic relations, alignment and comparison, BLAST, gene identification, synteny, comparative genomics). From the sequence of genomes to the function of genes. Forensic medicine (Biological sources of evidence, isolation, quantification and use of genetic material, SNP and STR markers, identification, analysis and use of marker polymorphisms, paternity-kinship study, mitochondrial DNA, Y chromosome).

Course objectives

The objectives of the course for students are to understand basic concepts of Molecular Biology related to the flow of information and the nature of genetic material. In particular, to develop analytical and critical analysis and synthesis skills. Understand important molecular mechanisms by emphasizing their regulation, and not memorize details of minor importance.

In addition, the aim for students is to get acquainted with the principles with which the most important cutting-edge techniques in Molecular Biology and to understand the applications of these techniques on basic and applied research.

It is also important to get students familiarized with the applications of Molecular Biology and Genetics in forensics.

Finally, the course introduces students to the modern and rapidly evolving field of Genomics, and in particular to applications related to human health, biotechnology and personalized medicine. Students are still familiar with the use of genomic databases and are concerned about the ethical and social dimensions of genomic research.

At the end of the course the students:

• will be familiar with the terms of Molecular Biology,

• will be familiar with a range of basic methods and techniques used in Molecular Biology,

• will be familiar with the interpretation of Molecular Biology experimental results and their evaluation through the prism of Genetics and Genomics research,

• will have understood the practical applications of these techniques in various fields such as Health,

• will have developed experimental design, formulation and control skills in Molecular Biology and Genetics

Textbooks/Bibliography

1. Burton E. Tropp. Βασικές Αρχές Μοριακής Βιολογίας. Jones & Bartlett Learning.
Ακαδημαϊκές Εκδόσεις, 2014.
1. James D. Watson, Jan A. Witkowski, Richard M. Myers, Amy A. Caudy. Ανασυνδυασμένο
DNA. Ακαδημαϊκές Εκδόσεις Ι. Μπάσδρα & ΣΙΑ Ο.Ε., 2007
1. Geoffrey M. Cooper & Robert E. Hausman. Το Κύτταρο: Μια Μοριακή Προσέγγιση.
Ακαδημαϊκές Εκδόσεις Ι. Μπάσδρα & ΣΙΑ Ο.Ε, Αλεξανδρούπολη, 2011.
1. Lewin. Genes VIIΙ. Ακαδημαϊκές Εκδόσεις Ι. Μπάσδρα & ΣΙΑ Ο.Ε, Αλεξανδρούπολη, 2004
2. Lisa Seidman. Εργαστηριακοί υπολογισμοί στις βιολογικές επιστήμες. Ακαδημαϊκές
Εκδόσεις Ι. Μπάσδρα & ΣΙΑ Ο.Ε., 2010
1. Peter J. Russell. iGenetics – Μια Μεντελική Προσέγγιση. Ακαδημαϊκές Εκδόσεις, 2009
2. Διονύσιος Κ. Μουρελάτος. Στοιχεία Εφαρμοσμένης Γενετικής του Ανθρώπου, University
Studio Press, 2009
1. Μαρία Γεωργίου. Γενετική Ιατροδικαστική. Εκδόσεις Παρισιάνου ΑΕ, 2008
2. Brown Τ. Α .Γονιδιώματα – σύγχρονες ερευνητικές προσεγγίσεις. BROKEN HILL PUBLISHERS
LTD, Αλεξανδρούπολη, 2010
1. S.H.Y. WONG, M.W. LINDER, R. VALDES. Φαρμακογονιδιωματική και πρωτεϊνωματική: Τα
εργαλεία της εξατομικευμένης ιατρικής, ΠΑΡΙΣΙΑΝΟΥ ΑΝΩΝΥΜΗ ΕΚΔΟΤΙΚΗ, 2010